Info-enriched characterization of cell populations. Morphological information and facts from photographs can include to your richness of flow cytometry knowledge. For instance, the determine displays an normally standard apoptosis assay using Annexin V and PI, adding mobile imaging to characterize cells in Every single inhabitants to expose morphologically distinctive capabilities. These insights couldn't have already been received from multiplex staining alone.

As samples are obtained within the Attune CytPix Flow Cytometer, the substantial-speed brightfield digital camera captures and outlets illustrations or photos of detected events at a fee as much as six,000 images per 2nd, according to the flow charge and impression sizing. For higher versatility, Attune Cytometric Computer software allows you to regulate the picture seize frequency as desired and choose a specific gate for impression capture, if required.

Mix acoustic concentrating know-how with superior-parameter Evaluation for versatile spectral unmixing and standard workflows.

Discover new insights into your experimental details by visualizing populations making use of blended fluorescence and morphology info. Our software interface wizards allow downsampling, concatenation (merged documents) and dimensionality equipment which can be constructed to the software package for an intuitive, streamlined data Assessment working experience.

*Pricing is indicative only which is subject matter to change. Be sure to contact your Agilent agent with any queries. The knowledge set forth herein isn't an offer or a determination, the two of which might be subject to credit approval, stipulations, and availability with Agilent. Agilent Fiscal Methods is available in picked nations.

On top of that, it's illuminating to find out what number of doublets slip via common singlet gates, and fantastic to confirm the standing of unexpected double-positive gatherings in my Assessment."

Optimizing gating system. Even strong handbook singlet gating is mistake-inclined and stays a subjective determination issue in almost all flow cytometry assays. Imaging can be used to verify and change gates to include only one cells of fascination.

Steady graphic good quality even at significant throughput Acoustic concentrating plus a superior-velocity digital camera Blend to graphic these T cells consistently at lower or high flow costs. Simply alter concentration and digicam configurations to satisfy experimental needs.

Early signs of this contamination experienced initially been dismissed as particles. Backgating to find new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also enables you to use morphological functions to find fascinating subpopulations that might not be evident from flow cytometry facts by yourself.

Maybe most of all, these occasions consist of cells of clearly distinct phenotypes which could lead on to incorrect conclusions relating to double favourable gatherings (especially in unusual populations).

AI-pushed automatic image Investigation with the Attune Cytometric Program translates function options into distinctive morphology parameters which can be combined with regular fluorescence and scatter parameters to reveal extremely reproducible inhabitants modifications and unique subsets.

We previously demonstrated the power of imaging Automobile-T/Ramos mobile interactions. Permit’s have a look at just the populace of greatest desire, the double good functions, To find out more. We will now use prolonged morphological parameters (here, circularity and skewness of intensity) to further more study the options of such populations and refine gating on these occasions, check here raising knowledge robustness.

Constructed-in dimensionality reduction equipment enhanced resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to verify anticipated population resolution in lysed complete human blood. User can watch functions from Every single cluster for visual confirmation of cell phenotypes.

No clean/no lyse leukocyte Evaluation with imaging. Cells were obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Skilled UX/UI allows end users to educate AI models for expanded apps. (A) The “Teach Design” feature supplies annotation tools and real-time design teaching accuracy.

At the center of each Attune Flow Cytometer lies our revolutionary acoustic concentrating technology, which makes use of seem waves to meticulously align cells within the flow cell. This modern solution surpasses standard hydrodynamic techniques, resulting in speedier processing speeds, Extraordinary information high quality, and amplified sample throughput.

The Attune Xenith cytometer is often a high-parameter spectral analyzer, giving each spectral unmixing and traditional flow capabilities for in depth and functional cell analysis.

Agilent Worth Promise guarantees you a minimum of 10 years of instrument use from the day you purchase. Normally, We're going to credit score you With all the residual price of the program toward an upgraded product.

Listed here, we display that by gating procedures applying picture-centered quantitative parameters can distinguish interacting cells from coincident activities to extra correctly analyze interacting cells.